We have discussed
the general purpose instruments used in chemical and microbiological
laboratories and also the instruments used to determine the physical and
rheological properties of food and other substances. Sophisticated modern equipments/
instruments which can determine very low concentration of constituents with
great precision like rancimate, GLC, TLC, HPLC, GCMS and LCMS have also been
discussed. However, these instruments are of general use and can be used in any
laboratory for example in pharmaceutical, forensic laboratory, food laboratory
etc. On the other hand, there are some instruments which are specifically designed
to be used in a dairy laboratory to determine the physico-chemical properties
of milk and milk products. Some of these instruments are discussed below.
i.
Gerber Centrifuge: Gerber centrifuge is used for the determination of fat in milk
and milk products. This centrifuge is different from other centrifuge. It has provision
to hold special glass tubes known as butyrometers, time adjustment clock and
rotate at fixed speeds.
ii.
Lactometer: Lactometer is a special type of hydrometer used for the determination
of specific gravity of milk and to calculate the total solids and solid not fat
(SNF) in milk. There are different types of lactometers such as zeal lactometer,
Quevene type of lactometer and ISI lactometer etc. Lactometer consist a slender
glas stem of uniform diameter connected to a large glass chamber, this chamber
causes the instrument to float. At the lower end of the body is a glass bulb
filled with lead shots or mercury which enable the instrument to sink to a
proper level and also to float. Depending upon the specific gravity of milk the
extent of sinking of the lactometer gives an idea of the purity of milk.
iii.
Milko Tester: There are 3 models mark II, mark III and automatic. The instrument
is used for fat determination in milk. It can analyse 80-120 samples/ hour and
require very small sample of milk (1.6 ml). The instrument gives results which
are comparable to reference method. The instrument determine the fat content on
the basis of scattering of light by a diluted sample of milk. Calcium chelating
agent eliminate the turbidity caused by the casein micelles, hence the scattering
of light is due to the presence of fat globules. The size and number of fat
globules influence the result therefore the sample is homogenized in a 4-stage homogenizer
to reduce the size of the globules to a specific size.
iv.
Electronic Milk Tester with Digital Read out: This
instrument is manufactured in the country by the Rajasthan Electronic and
instrument Ltd Jaipur. This is a electronic instrument used for determination
of fat content of milk.The determination follows the well known principle of
dilution, mixing,homogenization and photometric measurement of light scattered
by the fat globules present in
milk sample. Fat content is displayed quickly and accurately on a digital
readout.
v.
Infra Red Milk Analyzer (Milko Scan) Lactoscope: This
instrument is used for simultaneous determination of Fat, Protein, solid not
fat (SNF) and lactose in milk. The infrared milk analyzer measures absorption
of infrared energy by carbonyl groups at 5.7 in the easter linkage of fat
molecules, by peptides (at 6.46) linkages of amino acids in protein molecules,
and by hydroxyl groups of lactose molecules (9.6). The SNF is determined by
putting a constant to instrument
values for protein and lactose. In 1975, Foss Electric co. intdouced the single
cell, dual wavelength infrared milk analyzers (milko scan 203 and 300). In
these instruments optical filters isolate the specific wave lengths absorbed by
fat, protein and lactose and reference wave lengths not absorbed by these
components, thus eliminating the need for a diffraction grating. The 100 series
milko scan are the improved version of earlier milk scan. In this series milko
scan 133-B is the latest micro processor controlled instrument.
vi.
Lacto star Automatic Milk Analyzer: Lactostar-9 new,
versatile, esy to operate, microprocessor based, fully automatic milk analyzer
determines fat,SNF, proteins, lactose and freezing point of milk. The
instrument is introduced in India by Funke Gerber, Germany. It applies a
combined thermo-optical method to analyze the milk constituents. It determine
the sum of fat and proteins by optical measurement (Turbidemetric), while fat
and SNF content are determined by thermal analysis, whereas the lactose and
proteins are determined by computationed analysis. The freezing point is
predicted on the basis of protein and SNF content.
vii.
Lactoscope: Lactoscope from Delta instrument determines fat, proteins,lactose,
SNF and total solids in milk and milk products. The instrument is very
sophisticated and built for self-service so that any maintenance that is required
can be carried out via a modem connected to the manufacture’s head office.
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